Abstract
Angiogenesis is the formation of new blood vessels that plays a key role in wound healing, reproduction, embryonic vascular development, and replacement of damaged body tissues; however, it is also present in pathophysiological conditions such as cancer and cardiovascular diseases, reasons like these demand for more researches that aim for cure and prevention. This where anti-angiogenesis comes into the picture as it inhibits the above-mentioned conditions. Medicinal plants like, Ficus benjamina and Carmona retusa that are commonly found in gardens were observed to have these anti-angiogenic properties. In this study, various concentrations of Ficus benjamina and Carmona retusa leaf extracts were applied on Anas platyrhynchos Chorioallantoic Membrane as it exhibited the essential features of angiogenesis. Each leaf extracts was conducted to a control group with three different concentrations (100mg/disc, 10mg/disc, and 1mg/disc); Retinoic acid being the positive control and distilled water was used as a negative control. To measure the anti-angiogenic effect of each treatment on the CAM, ImageJ, a software was used to count the number of blood vessel branches and its length. One-way ANOVA and Tukey-HSD were used to analyze the results. It showed that the treatment with the highest concentration of Ficus benjamina inhibits angiogenesis compared to other concentrations and treatments with Carmona retusa. There was a significant difference among the different treatments in terms of the number of blood vessels branches and branches’ length. Such results stated that both leaf extracts inhibit angiogenesis. All inferential tests were set at 5% alpha level of significance.